Regulatory

Part:BBa_K5471005:Design

Designed by: Michail Diamantakis   Group: iGEM24_Patras-Med   (2024-09-29)


EF1a promoter (+EF1a intron)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 452
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 452
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 600
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 452
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 452
    Illegal NgoMIV site found at 335
    Illegal AgeI site found at 60
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was excised from an already existing plasmid backbone and was immediately ready for use


Source

This part is directly taken from plasmid "scAAV GFP" and specifically is comprised of the region from 155 to 958 (from 5' end)

scAAV GFP was a gift from K. Reed Clark (Addgene plasmid # 21893)

References

https://www.addgene.org/21893/

Therapeutic microRNA delivery suppresses tumorigenesis in a murine liver cancer model. Kota J, Chivukula RR, O'Donnell KA, Wentzel EA, Montgomery CL, Hwang HW, Chang TC, Vivekanandan P, Torbenson M, Clark KR, Mendell JR, Mendell JT. Cell. 2009 Jun 12. 137(6):1005-17. 10.1016/j.cell.2009.04.021 PubMed 19524505